报告题目：Transcriptional Engineering Tools for E. coli
报 告 人：Dr. Rongrong Jiang
Abstract：Breeding microorganisms with desired properties has often been achieved by classical strain engineering methods, which includes spontaneous adaptation and use of UV/chemical mutagens, and metabolic engineering approach. However, classical strain engineering methods are usually time-consuming and labor-intensive, whereas metabolic engineering approach requires detailed metabolic information.
In recent years, transcriptional engineering has started to attract attention in strain engineering. Here, we try to improve strain performance under stress by manipulating its transcription profile. Our target is global regulator cAMP receptor protein (CRP), which can regulate over 400 genes in E. coli. Directed evolution methods were employed to introduce modifications to CRP and the mutant library was subjected to selection based on cell growth under various stresses. With this approach, we have successfully isolated E. coli variants with improved oxidative stress tolerance, osmotolerance, biofuel tolerance (1-butanol, isobutanol, ethanol), organic solvent tolerance, acetate stress tolerance and low pH tolerance.